Anti-O-Linked N-Acetylglucosamine antibody [RL2] ab2739 from Abcam

Product Specs
ItemAnti-O-Linked N-Acetylglucosamine antibody [RL2]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2739
Antibody TypePrimary
ClonalityMonoclonal
HostMouse, Nude mouse
CloneRL2
Quantity10 UG, 100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot, Immunocytochemistry/ Immunofluorescence, Western blot
IsotypeIgG1
Conjugate/TagUnconjugated
ReactivityHuman, Rat
TargetO-Linked N-Acetylglucosamine

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylationNature chemical biologyJanuary 1, 2024Chen L, Zhou Q, Zhang P, Tan W, Li Y, Xu Z, Ma J, Kupfer GM, Pei Y, Song Q, et al.γ-H2AX (S139) (Cell Signaling Technology, 9718); histone H3 (Millipore, 06-755); O-GlcNAc (RL2) (Abcam, ab2739); O-GlcNAc (CTD110.6) (Cell Signaling Technology, 9875); O-GlcNAc (18B10.C7) (Thermo Fisher Scientific,

(2) O-GlcNAcylation regulates HIF-1α and induces mesothelial-mesenchymal transition and fibrosis of human peritoneal mesothelial cellsHeliyonDecember 1, 2023Jian Wang, Xin Lv, Ashanjiang Aniwan, Hongyan Liu, Yao Lin, Xian Shao, Saijun Zhou, Pei Yumethods 2.1 Antibodies & chemicals The antibodies against O-linked N-acetylglucosamine (O-GlcNAc) (RL2, ab2739) were purchased from Abcam. Anti-OGT (F-12,sc-74546), anti- O-GlcNAcase (OGA) (G-12,sc-376429), anti–HIF–1α (28b

(3) O-GlcNAcylation is essential for therapeutic mitochondrial transplantationCommunications MedicineNovember 25, 2023Ji Hyun Park, Masayoshi Tanaka, Takafumi Nakano, Ester Licastro, Yoshihiko Nakamura, Wenlu Li, Elga Esposito, Emiri T. Mandeville, Sherry Hsiang-Yi Chou, MingMing Ning, et al.(1:500, Santacruz, sc-7049), anti-TOM40 (1:200, Santacruz, sc-11414), anti-O-GlcNAc (1:1000, Abcam, ab2739), anti-DJ1 (1:1000, Cell signaling, 5933S), anti-Atg12, anti-Atg5, and anti-LC3A/B (1:1000, Cell signaling,

(4) O-GlcNAcylation regulates OTX2's proteostasisiScienceNovember 17, 2023Wulff-Fuentes E, Boakye J, Kroenke K, Berendt RR, Martinez-Morant C, Pereckas M, Hanover JA, Olivier-Van Stichelen S.13497-1-AP; RRID:AB_2157176 Mouse monoclonal anti-O-Linked N-Acetylglucosamine antibody (RL2),Abcam,Cat# ab2739; RRID:AB_303264 Rabbit polyclonal anti-Actin,Millipore Sigma,Cat# A2066; RRID:AB_476693 Mouse monoclonal

(5) O-GlcNAc has crosstalk with ADP-ribosylation via PARGJournal of Biological ChemistryNovember 1, 2023Jie Li, Xiangxiang Liu, Bin Peng, Tingting Feng, Wen Zhou, Li Meng, Shanshan Zhao, Xiyuan Zheng, Chen Wu, Shian Wu, et al.PARP1 (20) and PARG (36) plasmids were previously described. Antibodies were as follows: RL2 (Abcam, AB2739), anti-c-Myc (ProteinTech, 10828-1-AP), anti-PARG (Santa Cruz, Sc-398563), anti-DDB1 (GeneTex, GTX100130),

(6) H3K4me3 remodeling induced acquired resistance through O-GlcNAc transferase☆Drug Resistance UpdatesNovember 1, 2023Dinoop Ravindran Ravindran Menon, Heinz Hammerlindl, Gregory Gimenez, Sabrina Hammerlindl, Elmar Zuegner, Joachim Torrano, Natalie Bordag, Abdullah Al Al Emran, Maybelline Giam, Simon Denil, et al.to 500 μg total protein were mixed with 2 μL Anti-O-Linked N-Acetylglucosamine antibody [RL2] (Abcam ab2739) and incubated overnight at 4°C with gentle rocking. Immunoprecipitation was performed by adding 10

(7) Cryo-EM structure of human O-GlcNAcylation enzyme pair OGT-OGA complexNature CommunicationsOctober 31, 2023Ping Lu, Yusong Liu, Maozhou He, Ting Cao, Mengquan Yang, Shutao Qi, Hongtao Yu, Haishan GaoTAB1/YTHDF1 O-GlcNAcylation was verified by Western blotting using the anti-O-GlcNAc antibody (RL2) (Abcam, ab2739). The peak fractions containing G-TAB1/YTHDF1 were collected and stored. Pull-down assays For GST

(8) Reciprocal regulation of TWIST1 and OGT determines the decitabine efficacy in MDS/AMLCell Communication and SignalingSeptember 22, 2023Hongjiao Li, Yi Wang, Shuang Feng, Kaijing Chang, Xinwen Yu, Fenfang Yang, Haozhe Huang, Yuanbo Wang, Xiang Li, Feng Guanthe plate was washed three times with 200 μL 0.05% Tween 20 in PBS. The antibody against O-GlcNAc (#ab2739, Abcam, Cambridge, MA, USA) and the secondary antibody conjugated horseradish peroxidase (HRP) was added

(9) Hepatocyte-specific O-GlcNAc transferase downregulation ameliorates nonalcoholic steatohepatitis by improving mitochondrial functionMolecular MetabolismSeptember 1, 2023Maria J. Gonzalez-Rellan, Tamara Parracho, Violeta Heras, Amaia Rodriguez, Marcos F. Fondevila, Eva Novoa, Natalia Lima, Marta Varela-Rey, Ana Senra, Maria D.P. Chantada-Vazquez, et al.0.25% BSA) in TBS 0.1 M for 60 min. Next, sections were incubated with the primary antibodies O-GlcNac (ab2739, Abcam) (1:200) and Albumin (ab8940, Abcam) (1:200) in blocking solution for 24 h at 4 °C. After incubation

(10) O‑GlcNAcylation mediates endometrial cancer progression by regulating the Hippo‑YAP pathwayInternational journal of oncologyAugust 1, 2023Zhai L, Yang X, Dong J, Qian L, Gao Y, Lv Y, Chen L, Chen B, Zhou F.were as follows: Anti-OGT (1:1,000; cat. no. ab96718, Abcam), anti-O-GlcNAcylation (1:1,000; cat. no. ab2739, Abcam), anti-YAP (1:2,000; cat. no. 13584-1-AP, Proteintech Group, Inc.), anti-phosphorylated (p-)YAP

(11) Inhibition of DNMT1 methyltransferase activity via glucose-regulated O-GlcNAcylation alters the epigenomeeLifeJuly 20, 2023Shin H, Leung A, Costello KR, Senapati P, Kato H, Moore RE, Lee M, Lin D, Tang X, Pirrotte P, et al.monoclonal),Abcam,Cat# ab18185,IP (1:250)WB (1:1000) Antibody,Anti-O-GlcNAc (RL2)(mouse monoclonal),Abcam,Cat# ab2739,WB (1:1000) Antibody,Aoat anti-rabbit IgG H&L (HRP)(goat polyclonal),Abcam,Cat# ab6721,WB (1:5000) Antibody,Goat anti-mouse IgG H&L (HRP

(12) Blockage of autophagosome-lysosome fusion through SNAP29 O-GlcNAcylation promotes apoptosis via ROS productionAutophagyJuly 3, 2023Francesca Romana Pellegrini, Sara De Martino, Giulia Fianco, Irene Ventura, Davide Valente, Mario Fiore, Daniela Trisciuoglio, Francesca Degrassiand its O-GlcNAcylation levels were analyzed by immunoblot using an antibody against O-GlcNAc (Abcam, ab2739) and an anti-SNAP29 antibody (Santa Cruz Biotechnology, sc-390,602). Statistical analysis Experiments

(13) Castration-resistant prostate cancer cells are dependent on the high activity of CDK7Journal of Cancer Research and Clinical OncologyJuly 1, 2023Satu Pallasaho, Aishwarya Gondane, Anni Kuivalainen, Samuel Girmay, Siver Moestue, Massimo Loda, Harri M. Itkonen(ThermoFisher Scientific) according to the manufacturer’s protocol and combination of two antibodies: RL2 (Abcam: ab2739) and CTD110.6 (Cell Signaling Technologies: 9875). Cell lysis buffer was supplemented with protease

(14) Restoration of coronary microvascular function by OGA overexpression in a high-fat diet with low-dose streptozotocin-induced type 2 diabetic miceDiabetes and Vascular Disease ResearchMay 15, 2023Jody Tori Cabrera, Rui Si, Atsumi Tsuji-Hosokawa, Hua Cai, Jason X-J Yuan, Wolfgang H Dillmann, Ayako Makinomembranes. The following primary antibodies were used: RL2 (an anti-O-GlcNAc antibody, 1:5000, Abcam, #AB2739) and anti-actin (a loading control. 1:5000, Cell Signaling, #4968). RL2 binds to O-GlcNAc and is used

(15) Spatial mapping of mitochondrial networks and bioenergetics in lung cancerNatureMarch 23, 2023Mingqi Han, Eric A. Bushong, Mayuko Segawa, Alexandre Tiard, Alex Wong, Morgan R. Brady, Milica Momcilovic, Dane M. Wolf, Ralph Zhang, Anton Petcherski, et al.GT11-A, Alpha Diagnostic); NDUFS1 (1:1,000, ab169540, Abcam); O-linked N-acetylglucosamine (1:1,000, ab2739, Abcam); SDHA (1:1,000, 5839, Cell Signaling Technology); SDHC (1:1,000, ab155999, Abcam); actin (1:5,000,

(16) CircVPRBP inhibits nodal metastasis of cervical cancer by impeding RACK1 O-GlcNAcylation and stabilityOncogeneMarch 10, 2023Chunyu Zhang, Hongye Jiang, Li Yuan, Yuandong Liao, Pan Liu, Qiqiao Du, Chaoyun Pan, Tianyu Liu, Jie Li, Yili Chen, et al.(1:1000, Cat# ab177941, Abcam), rabbit anti-O-linked N-Acetylglucosamine antibody [RL2] (1:1000, Cat# ab2739, Abcam), mouse anti-flag antibody (1:5000, Cat#F3165, Sigma-Aldrich), or rabbit anti-HA (1:1000, Cat#

(17) Glutamine is a substrate for glycosylation and CA19-9 biosynthesis through hexosamine biosynthetic pathway in pancreatic cancerDiscover OncologyFebruary 17, 2023Chen Liu, Shengming Deng, Zhiwen Xiao, Renquan Lu, He Cheng, Jingjing Feng, Xuxia Shen, Quanxing Ni, Weiding Wu, Xianjun Yu, et al.obtained. After blocking with 5% BSA (Yeasen, Shanghai) in PBS, antibodies against O-GlcNAc (Abcam, Inc., ab2739) or CA19-9 (Abcam, Inc., ab3982) at a dilution of 1/200 in 5% BSA were used to detect their expression

(18) O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N(6)-methyladenosine-dependent mannerSignal transduction and targeted therapyFebruary 10, 2023Yang Y, Yan Y, Yin J, Tang N, Wang K, Huang L, Hu J, Feng Z, Gao Q, Huang A.are provided in Supplementary Table 1. Antibodies Antibodies against YTHDF2 (ab220163), O-GlcNAc (ab2739), OGA (ab124807), OGT (ab96718), K48 (ab271911) and p-Rb (ab173289) were obtained from Abcam (Cambridge,

(19) Glutamine promotes O-GlcNAcylation of G6PD and inhibits AGR2 S-glutathionylation to maintain the intestinal mucus barrier in burned septic miceRedox biologyFebruary 1, 2023Wu D, Su S, Zha X, Wei Y, Yang G, Huang Q, Yang Y, Xia L, Fan S, Peng X.antibodies against G6PD (Abcam, ab993), CHOP (Abcam, ab11419), AGR2 (Abcam, ab76473), O-GlcNAC (Abcam, ab2739), glutathione (Virogen, 101-A), GRP78 (Cell Signaling, 3177S), or GAPDH (Aksomics, KC-5G4). The appropriate

(20) Polo-like kinase 1 (PLK1) O-GlcNAcylation is essential for dividing mammalian cells and inhibits uterine carcinomaJournal of Biological ChemistryFebruary 1, 2023Sheng Yan, Bin Peng, Shifeng Kan, Guangcan Shao, Zhikai Xiahou, Xiangyan Tang, Yong-Xiang Chen, Meng-Qiu Dong, Xiao Liu, Xingzhi Xu, et al.antibodies were described before (13). PLK1 antibodies were Santa Cruz, sc-17783; RL2 antibodies were Abcam, AB2739. PLK1 mutant plasmids were generated using specific primers (sequences available upon request) following

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3/30/2020Easy-to-Use and Sensitive Antibody for O-Linked N-Acetylglucosamine Wei Yang SitA easy to use and sensitive antibody. It is highly recommended to those who need it.

Anti-O-Linked N-Acetylglucosamine antibody [RL2] from Abcam

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Anti-O-Linked N-Acetylglucosamine antibody [RL2] from Abcam

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